动物造模,抑郁症 z-bio 2023-09-07 752

　　Objective: To investigate whether lipid metabolism disorders in postmenopausal women are involved in the formation of depressive symptoms.

　　Method: 24 LDLR -/- and 16 WT C57BL/6J female mice were divided into 5 groups (n=8), namely: WT+regular diet group (WT), WT+high fat diet group (WT-H), LDLR -/- ovariectomized+regular diet group (LDLR -/-), LDLR -/- ovariectomized+high fat diet group (LDLR -/- H), LDLR -/- ovariectomized+high fat diet+simvastatin group (XF), and fed continuously for 3 months. Establishment of a postmenopausal lipid metabolism disorder model in LDLR -/- female mice with bilateral ovariectomy combined with a high-fat diet. Detection of mouse body weight, brain total cholesterol (TC), and hippocampal ER α、 ER β Level, depressive behavior, and brain 5-HT levels; Conduct correlation analysis between brain TC levels and depression related indicators.

　　Result (1) The LDLR -/- H group could replicate the characteristics of postmenopausal lipid metabolism disorders, manifested as significant weight gain in mice, significant increase in brain TC levels, and hippocampal ER β Significant decrease in expression; (2) The LDLR -/- H group mice showed a significant decrease in both horizontal and vertical movements, a significant increase in TST rest time, and a significant decrease in brain 5-HT levels; (3) The XF group mice showed a significant decrease in brain TC levels, improvement in depressive behavior, and a significant increase in 5-HT levels; (4) The TC level in the mouse brain is significantly correlated with 5-HT levels and depressive behavior.

　　Conclusion: Postmenopausal lipid metabolism disorder can induce the occurrence of depressive symptoms, and regulating lipid metabolism can significantly improve depressive symptoms.