动物造模,药效评价 中国实验动物信息网 2024-11-21 864

　　Objective: To observe the effect of Huangyangning on oxidative stress levels in dogs with atrial frontal movement.

　　Method 15 beagles were randomly divided into 5 groups: blank control group, model group, sham operation group, Huangyangning group (0.24mgkg), and Probucol group (7.58mgkg). A canine atrial frontal model was constructed by inserting a human bipolar electrode into the right atrial appendage of the dog through dice vein puncture, with a high-frequency pacemaker connected to the tail and high-frequency stimulation given. Model success criteria: After one week of pacing, if the electrocardiogram confirms the occurrence of atrial fibrillation or triggers atrial frontal seizures during the programmed process, the model is considered successful. Start gastric lavage 1 day after surgery, atrial pacing 1 week after surgery, and sample collection 4 weeks later. Observing changes in myocardial fibers with Masson staining: ELISA detection of angiotensin II (Agl), reduced nicotinamide adenine dinucleotide phosphate (NADPH) serum content; chemiluminescence detection of reactive oxygen species (ROS) content; colorimetric determination of total antioxidant capacity (TAC); RT-PCR, Western blot determination of NADPH oxidase (Nox) subunit gene p22, P47 expression levels.

　　Compared with the blank group, the serum AngI and NADPH levels in the model group were significantly increased (P<0.05), and the levels of R0S/TAC were significantly increased (P<0.05). The expression of P22P47 gene was significantly increased, while the expression of P22 and p47 protein was also increased. After the intervention of Huangyangning and Probucogan, there was no significant increase in serum NADPH levels: R0S/TAC levels were lower than the model group, with a significant difference (P<0.05): The expression of Rac-1P22P47 gene was lower than the model group, with statistical significance (P<0.05): The expression of P22 and P47 protein was lower than the model group, with significant statistical significance (P<0.05).

　　Conclusion: Huangyangning can alleviate oxidative stress levels in dogs with atrial fibrillation, and its mechanism of action may be related to the Ag-N0x-R0S signaling pathway.