动物造模,脾胃虚弱型功能性消化不良,药效评价 中国实验动物信息网 2024-11-15 921

　　Objective: To explore the intervention mechanism of Xiangsha Liujunzi decoction on functional dyspepsia rats with spleen and stomach weakness.

　　Method: Sixty SPF grade male SD lactating rats were randomly divided into a blank group and a modeling group. The modeling group rats were used to replicate the spleen stomach weakness type FD rat model using a comprehensive modeling method (iodoacetamide perfusion+swimming exhaustion+hunger and satiety disorders). After successful replication of the model, the rats in the modeling group were randomly divided into 5 groups: model group, positive control group, and high, medium, and low dose groups of Xiangsha Liujunzi Tang, with 10 rats in each group. The blank group and model group rats were given 10mL/(kg · d) physiological saline by gavage, while the positive control group was given 1 35mg/(kg · d) Mosapride was administered by gavage, and the high, medium, and low dose groups of Xiangsha Liujunzi Tang were given 12, 6, and 3g/(kg · d) Xiangsha Liujunzi Tang by gavage, with continuous intervention for 14 days. Observe the general condition of rats before and after modeling and administration, measure their body weight and 3-hour food intake. After the administration intervention, the gastric emptying rate and intestinal propulsion rate of rats were measured. The pathological changes of gastric tissue were observed using hematoxylin eosin staining, the content of Mg2+⁃ ATPase in rat gastric tissue homogenate was measured using a biochemical reagent kit, and the expression levels of CaM, MLCK, and p ⁃ MLC20 proteins in gastric tissue were detected using protein immunoblotting.

　　Compared with the blank group, the model group showed a significant decrease in hair loss, fatigue, lethargy, and delayed movement, as well as a significant decrease in general condition scores, as well as a significant decrease in body weight, 3-hour food intake, gastric emptying rate, and intestinal propulsion rate (P<0.05); No obvious abnormalities were found in the pathology of gastric tissue; The content of Mg2+⁃ ATPase in gastric tissue decreased, and the expression levels of CaM, MLCK, and p ⁃ MLC20 proteins in gastric tissue were significantly reduced (P<0.05). Compared with the model group, the general condition scores of rats in the high and medium dose administration groups were significantly improved, and their body weight, 3-hour food intake, and gastric emptying rate were significantly increased (P<0.05); There was no significant difference in gastric tissue pathology; The content of Mg2+⁃ ATPase in gastric tissue significantly increased, and the expression levels of CaM, MLCK, and p ⁃ MLC20 protein in gastric tissue significantly increased (P<0.05). The intervention effect of the Xiangsha Liujunzi Tang group on the above indicators was dose-dependent.

　　Conclusion: Xiangsha Liujunzi Decoction can effectively improve the general condition and gastric motility of FD rats with spleen stomach weakness. The mechanism of action may be related to its activation of the CaM/MLCK/MLC20 pathway in gastric tissue to regulate smooth muscle contraction and promote gastric motility.