动物造模,肠缺血再灌注损伤,药效评价 中国实验动物信息网 2024-11-12 985

　　Objective: To evaluate the impact of small intestinal ischemia time on small intestinal ischemia-reperfusion models from the perspective of oxidative damage pathways and histopathology, and to provide modeling conditions and basic data for the study of this disease.

　　Methods Twenty four male SD rats were randomly divided into Sham surgery group, 30 min ischemia group, 1 h ischemia group and 2 h ischemia group. The ischemia reperfusion injury model was induced by ligating the superior Mesentery artery for a certain time after ischemia and reperfusion for 4 h. The rats were euthanized 4 hours after the blood supply was restored. After 10% of the intestinal tissue was homogenized, the levels of Superoxide dismutase (SOD), Malondialdehyde (MDA), Glutathione peroxidase (GSH Px) and glutathione (GSH) in the small intestine were compared through the corresponding detection of the kit. The morphology of the intestinal tissue was observed by HE staining, and the villus height and mucosal thickness of the small intestine were measured.

　　Results Compared with the Sham surgery group, the levels of SOD, GSH Px and MDA in 30 min ischemia group and 1 h ischemia group were significantly higher (P<0 Ischemia for 30 minutes, 1 hour, and 2 hours can exacerbate inflammatory cell infiltration, and significantly increase pathological Chiu's score (P<0.05) In addition, ischemia for 1h and 2h could significantly reduce the villus height and mucosal thickness of small intestine (P<0.05) 01). But ischemia for 2 hours can cause animal death (mortality rate 33 3%).

　　Conclusion: The oxidation index and intestinal tissue morphology of the small intestine ischemia-reperfusion model established after 1 hour of ischemia and 4 hours of reperfusion are the most ideal. The small intestine ischemia-reperfusion model established under this condition is suitable for disease research and drug development.