动物造模 中国实验动物信息网 2023-06-13 734

　　Objective: This article aims to analyze the behavioral abnormalities and pathological changes in brain tissue of rats treated with Trim44 gene knockout, and explore the mechanism of action of Trim44 gene in the nervous system.

　　Method: Protein blotting and immunohistochemistry were used to detect the expression of TRM44 in brain tissue. TUEL staining was used to observe the apoptosis of neurons in Trim44 treated rats. Behavioral tests such as Y-maze, water maze, and food maze were used to observe the behavioral changes in treated rats. Protein blotting was used to detect abnormal expression of apoptosis related proteins.

　　The results showed that TRM44 was expressed in the cerebral cortex, and the efficiency of Trim44 removal in the brain tissue of rats was up to 67%. Trim44 removal caused a decrease in learning and memory abilities, neuronal apoptosis, and increased expression of active forms of caspase3 and caspase9 proteins in elderly rats.

　　Conclusion: Trim44 promotes neuronal apoptosis and impairs the learning and memory abilities of rats. This indicates the important role of Trim44 in the nervous system, and the Weichu model can also be used for in-depth mechanism exploration.