动物造模,酒精性脂肪肝 中国实验动物信息网 2023-06-13 839

　　Objective To analyze the changes of protein expression profile in liver Histone of mice with alcoholic fatty liver disease and the intervention effect of Resveratrol by Proteomics.

　　Methods The AFLD model of C57BL/6J mice was prepared by NIAAA method, and Resveratrol (400 mg/kg) was administered orally for 9 consecutive days. The 4D non-standard quantitative proteomics method was used to identify and quantify proteins in the liver tissues of mice in each experimental group. To simultaneously satisfy a multiple change of ≥ 1 5 or ≤ 0 67, and P<0 05. The differentially expressed proteins significantly up-regulated or down-regulated in the normal control group and the AFLD group, the AFLD group and the Resveratrol intervention group, the normal control group and the Resveratrol group were screened, and then GO classification of differentially expressed proteins, KEGG pathway enrichment and protein network interaction analysis were carried out.

　　The results identified and quantified 4513 and 3763 proteins, and screened 1228 differentially expressed proteins and 11 differentially co expressed proteins. Compared with the control group (P<0.05), 370 and 324 proteins were significantly down regulated and up regulated in the AFLD group, and 40 and 43 proteins were significantly up regulated and down regulated in the Resveratrol intervention group; Compared with the AFLD group (P<0 05), 224 and 227 proteins were significantly up-regulated and down regulated in the Resveratrol intervention group. 1228 differentially expressed proteins were involved in 40 biological processes annotated by GO, 36 cell compositions, 38 molecular functions, and 45 KEGG pathways. 11 differentially co expressed proteins were analyzed by GO, KEGG, and protein-protein network interactions. Among them, 9 differentially co expressed proteins involved 9 sub functions, 5 differentially co expressed proteins involved 5 signaling pathways, and 4 differentially co expressed proteins interacted with each other.

　　Conclusion After chronic alcohol intake, the protein expression profile of liver Histone in mice has changed significantly, including sulfotransferase family cytosolic 1B member 1 (Sult1b1), apolipoprotein A-IV (Apoa4), glycerol-3-phosphate acyltransferase 3 (Gpat3) The changes in the expression levels of four proteins, including epoxide Hydrolase 1 (Ephx1), are closely related to the occurrence of AFLD and the intervention of Resveratrol in AFLD.