动物造模,结节性甲状腺肿 中国实验动物信息网 2023-06-13 995

　　Objective To determine the thyroid localization of rats by using ultrasound technology, and analyze and evaluate the pathological changes and modeling degree of nodular Goitre model in rats, so as to provide technical methods for the diagnosis and evaluation of nodular Goitre and related Thyroid disease model in rats.

　　Methods Forty SPF Wistar rats aged 6~8 weeks, half male and half female, of which 20 rats served as the control group, and the rest rats were perfused with propylthiouracil (PTU) solution for 8 weeks to construct the model of nodular goiter. After the model was established, the thyroid Ultrasonic testing was performed on the rats, and the pathological examination was performed at the same time, and the results were compared with the ultrasound results.

　　Results Ultrasonic testing showed that the anterior and posterior, internal and external diameters of thyroid in the model group were significantly larger than those in the control group (P<0 001), at the same time, it can be seen that the gland of the model group rats is asymmetrically enlarged, with nodules in the lateral lobe of the thyroid without a capsule, and the boundary is relatively blurry and irregular, with scattered strong echoes of dot or strip like fibrous hyperplasia. The HE staining of the thyroid slices of the model group rats showed that the shape of thyroid tissue and the size of follicles were irregular, with significant differences. Epithelial cells proliferated significantly, arranged in disorder, and glia were compressed and unevenly distributed. The ultrasound and pathological tests of the model group rats were consistent with disease diagnosis.

　　Conclusion Ultrasonic testing can be used to evaluate the rat model of nodular Goitre in vivo, which is worth popularizing.