动物造模,代谢紊乱 中国实验动物信息网 2023-06-12 1001

　　Objective To explore the protective effect of intermittent fasting on metabolic disorder induced by Olanzapine in mice and its mechanism.

　　Methods C57BL/6J mice were randomly divided into 4 groups: Saline+Ad libitum, Saline+IF, Olanzapine+Ad libitum, Olanzapine+IF, 8 in each group. The intermittent fasting group adopted a 5:2 plan, which means fasting on Mondays and Thursdays of each week, and eating freely at other times for 12 weeks of intervention; Free feeding was used as the control of intermittent fasting (Control), and normal saline was used as the control of Olanzapine administration (Saline). The body mass, liver mass and periepididymal adipose tissue mass of mice in the Olanzapine treated group and the control group after intermittent fasting intervention were compared; At the same time, the levels of Glucose test#Fasting blood sugar, insulin and insulin resistance index (HOMA-IR) in the process of glucose metabolism in mice were measured by glucose oxidase method and radioimmunoassay, respectively. The content of H2O2 and the level of cytochrome C mRNA, a marker related to mitochondrial damage, were measured by ELISA and real-time fluorescent quantitative PCR, respectively.

　　Results After 12 weeks of grouping treatment, Olanzapine significantly increased the body weight, body fat, leptin and visceral fat infiltration in mice (P; However, intermittent fasting can significantly reduce the above indicators (P<0.05). Further research has shown that the release of H2O2 and the expression level of Cytochrome C mRNA in adipose tissue of mice after intermittent fasting treatment are significantly reduced (P<0.05).

　　Conclusion Intermittent fasting can alleviate the metabolic disorder induced by Olanzapine in mice, and its mechanism may be related to the inhibition of oxidative stress and the maintenance of mitochondrial function.