动物造模,阿霉素 中国实验动物信息网 2023-06-12 792

　　Objective To explore the effect and mechanism of Lingzhu Cusi Pill on adriamycin Nephrin.

　　Method: 36 male SD rats were included in the experiment. Randomly divide into 6 groups (6 animals/group). Including blank, model group, and PDTC (NF- κ B inhibitor group (100 mg/(kg · d)), as well as low, medium, and high dose groups of Lingzhu Tusi Pill (14, 28, 56 g/(kg · d)). Except for the blank group, all rats were treated with doxorubicin to create kidney disease models. After successful modeling, medication was administered continuously for 6 weeks. Record general vital signs; 24 h urinary protein, plasma albumin (Alb), serum creatinine (Scr), Blood urea nitrogen (BUN) and C-reactive protein (CRP) were collected and detected; HE, Masson, PAS staining, and transmission electron microscopy were used to observe the pathological morphological changes of the kidney; ELISA detection of serum IL-6 and TNF- α Horizontal; Western Blot detection of IKK β/ NF- κ B/MCP-1 pathway related proteins.

　　The survival status of the model group rats was poor. Alb decreases and 24-hour urine protein increases. Extensive swelling of renal tubules and fusion of glomerular foot processes; A lot of fiber deposition and Basement membrane thickening. Serum IL-6 and TNF- α And elevated CRP; Kidney p-IKK β、 P-NF- κ B. MCP-1 expression level and p-IKK β/ IKK β、 P-NF- κ B/NF- κ The B ratio increased, while the expression of Nephrin and Podocin decreased (P<0 05). Except for the high-dose group, the rats in the administration group were generally in good condition. Weight increases, 24-hour urine protein decreases, and Alb increases. The swelling of renal tubules is reduced, and the fusion of glomerular foot processes is improved. Fibrosis of renal tubules and glomeruli was reduced, and Basement membrane was not thickened. Serum IL-6 and TNF- α And a decrease in CRP; Kidney p-IKK β、 P-NF- κ B. MCP-1 expression level and p-IKK β/ IKK β、 P-NF- κ B/NF- κ The B ratio decreased, and the expression of Nephrin and Podocin increased (P<0 05).

　　Conclusion: Lingzhu Tusi Pill reduces 24-hour urinary protein and serum inflammatory factors in rats with adriamycin induced nephropathy, and its mechanism of action may be related to the inhibition of IKK β/ NF- κ B/MCP-1 signaling pathway.